Department of Biological Sciences

Indian Institute of Science Education and Research Mohali

(0172) 229 3152

headbiology@iisermohali.ac.in

Shravan Kumar Mishra

Associate Professor

Research

We use molecular biology, genetics, and biochemistry approaches in the budding yeast Saccharomyces cerevisiae and the fission yeast Schizosaccharomyces pombe to study functions of proteins related to ubiquitin in cell division, pre-mRNA splicing, heterochromatin formation and intracellular trafficking. A few ongoing projects are listed below:  Ubiquitin-like proteins in intron-specific splicing and alternative splicing: The process of pre-mRNA splicing through spliceosome is understood well. Its regulation is essential for intron-specific splicing and alternative splicing. We study the role of the ubiquitin-like proteins Hub1 and Sde2 in these processes.  Molecular evolution of the ubiquitin fold in Sde2: Multiple evidence suggests that the ubiquitin fold in the intron-specific pre-mRNA splicing factor Sde2 likely originated from ubiquitin. Can its origin be traced through experiments?  Function of AAA-ATPases: These enzymes are known to play modulatory roles in a large number of cellular machineries. We study their role in cell division.  Intracellular signalling from Golgi to the nucleus: How Golgi communicates with the nucleus? Is this communication mediated via intron-specific splicing, and whether ubiquitin-like proteins and deubiquitinating enzymes (DUBs) participate in the signalling? We study the roles of these molecules in the trafficking and degradation of transporters of nutrients, such as glucose and phosphate.  Deubiquitinating enzymes (DUBs) in cellular trafficking: The reversible process of ubiquitination plays a central role in regulating vesicular trafficking in the cell. Although the activities of E3 ubiquitin ligases that attach ubiquitin to protein substrates are well documented, the roles of DUBs that remove ubiquitin are yet to be studied.

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Education

2004-2012

Post-doctoral fellow

Ubiquitin-Like Proteins
1999-2004

PhD

Heat Stress Response in Tomato Plant
1997-1999

Masters of Science (MSc)

Biotechnology
1994-1997

Bachelor of Science (BSc)

Microbiology, Biochemstry

People

Sugata Chaudhuri B.Sc. Microbiology from St. Xavier's college, Kolkata

I am interested in studying the trafficking of plasma membrane nutrient transporters by a deubiquitinating enzyme complex.

Ankita Banyal M.Sc. Biotechnology from University of Allahabad

I study mechanism and regulation of alternative splicing using Saccharomyces cerevisiae

Amjadudheen VP BSMS from IISER Mohali

I study function of the Ubiquitin like protein Hub1 and regulation of pre-mRNA spliicing.

Mohd Khalid M.Sc. Zoology from University of Lucknow

I study function of a AAA-ATPase and its role in cell division.

Kanika Sharma M.Sc. Zoology from Kanoria College, Jaipur

I study roles of Deubiquitinating enzymes in protein trafficking using Schizosaccharomyces pombe.

Onika Kumari M.Sc. Biotechnology from Vinoba Bhave University, Hazaribagh

I study function of intron specific splicing regulators in Schizosaccharomyces pombe.

Itisha Sharma BSMS Student

Nandana Rajendran BSMS Student

P B Nishaant Kumar BSMS Student

I study molecular evolution of proteins from Ubiquitin

Teaching

Publication

Anil AT, Pandian R, Mishra SK 2024, Introns with branchpoint-distant 3′ splice sites: Splicing mechanism and regulatory roles. Biophysical Chemistry

Kolathur KK, Sharma P, Kadam NY, Shahi N, Nishitha A, Babu K, Mishra SK 2023, The ubiquitin-like protein Hub1/UBL-5 functions in pre-mRNA splicing in Caenorhabditis elegans. FEBS Letters

Anil AT, Choudhary K, Pandian R, Gupta P, Thakran P, Singh A, Sharma M, Mishra SK 2022, Splicing of branchpoint-distant exons is promoted by Cactin, Tls1 and the ubiquitin-fold-activated Sde2. Nucleic Acids Research

Sajeevan A, Pandian R, Mishra SK 2022, Vectors with a flexible multiple cloning site and modular epitope tags for gene expression studies in Schizosaccharomyces pombe. Gene Reports

Varikkapulakkal A, Ghosh A, Mishra SK 2022, Broader roles of the ubiquitin-like protein Hub1 indicated by its yeast two-hybrid interactors. micropublication Biology

Bachhawat AK, Pandit SB, Banerjee I, Anand S, Sarkar R, Mrigwani A, Mishra SK 2020, An inquiry-based approach in large undergraduate labs: Learning, by doing it the "wrong" way. Biochemistry and Molecular Biology Education

Singh A, Choudhuri P, Chandradoss KR, Lal M, Mishra SK, Sandhu KS 2020, Does genome surveillance explain the global discrepancy between binding and effect of chromatin factors?. FEBS Letters

Fragkostefanakis S, Simm S, El-Shershaby A, Hu Y, Bublak D, Mesihovic A, Darm K, Mishra SK, Tschiersch B, Theres K, Scharf C, Schleiff E, Scharf KD 2019, The repressor and co-activator HsfB1 regulates the major heat stress transcription factors in tomato. Plant, Cell & Environment

Thakran P, Pandit PA, Datta S, Kolathur KK, Pleiss JA, Mishra SK. 2018, Sde2 is an intron-specific pre-mRNA splicing regulator activated by ubiquitin-like processing. The EMBO Journal

Chanarat S, Mishra SK 2018, Emerging roles of ubiquitin-like proteins in pre-mRNA splicing. Trends in Biochemical Sciences

Mishra SK, Thakran P 2018, Intron specificity in pre-mRNA splicing. Current Genetics

Fragkostefanakis S, Mesihovic A, Simm S, Paupière MJ, Hu Y, Paul P, Mishra SK, Tschiersch B, Theres K, Bovy A, Schleiff E, Scharf KD 2016, HsfA2 Controls the Activity of Developmentally and Stress-Regulated Heat Stress Protection Mechanisms in Tomato Male Reproductive Tissues. Plant Physiology

Ammon T, Mishra SK, Kowalska K, Popowicz GM, Holak T, Jentsch S 2014, The conserved ubiquitin-like protein Hub1 plays a critical role in splicing in human cells. Journal of Molecular Cell Biology

Mishra SK, Ammon T, Popowicz GM, Krajewski M, Nagel RJ, Ares M, Holak T, Jentsch S 2011, Role of the ubiquitin-like protein Hub1 in splice-site usage and alternative splicing. Nature

Tripp J, Mishra SK, Scharf KD 2009, Functional dissection of the cytosolic chaperone network in tomato mesophyll protoplasts. Plant, Cell & Environment

Baniwal SK, Bharti K, Chan KY, Fauth M, Ganguli A, Kotak S, Mishra SK, Nover L, Port M, Scharf KD, Tripp J, Zielinski D, von Koskull-Doering P 2004, Heat stress response in plants: a complex game with chaperones and more than 20 heat stress transcription factors. Journal of Biosciences

Mishra SK, Tripp J, Winkelhaus S, Tschiersch B, Theres K, Nover L, Scharf KD 2002, In the complex family of heat stress transcription factors, HsfA1 has a unique role as master regulator of thermotolerance in tomato. Genes & Development

Nover L, Bharti K, Doering P, Mishra SK, Ganguli A, Scharf KD 2001, Arabidopsis and the heat stress transcription factor world: how many heat stress transcription factors do we need?. Cell Stress & Chaperones

Contact

Work Email: skmishra[at]iisermohali[dot]ac[dot]in Address: AB2-3F3, IISER Mohali, Department of Biological Sciences

Indian Institute of Science Education and Research (IISER)- Mohali
Knowledge City, Sector 81, SAS nagar 140306, INDIA
headbiology@iisermohali.ac.in| +91 172 2240266

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